Plasmid

Part:BBa_K4807019:Design

Designed by: Vaios Styliaras   Group: iGEM23_IOANNINA   (2023-10-11)


pET-29c(+)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 192
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 192
    Illegal BglII site found at 242
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
    Illegal NgoMIV site found at 436
    Illegal NgoMIV site found at 2024
    Illegal NgoMIV site found at 2184
    Illegal NgoMIV site found at 5231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3104


Design Notes

No changes made while designing this sequence.


Source

E.coli genome

References